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How to Calculate Sequence Coverage

Enrichment studies require the calculation of sequencing data to determine the level of coverage. This is done primarily with gene sequencing laboratories requiring integrated DNA sample preparation and pre-enrichment pooling. Calculating sequencing coverage requires using variables that include the mean sequencing coverage, the total targeted bases and the enrichment efficiency. Performing this calculation is done by inputting all of these variables into an equation.

Instructions

- 1
  Write out the equation used to calculate the mean sequencing coverage. This will look like the desired coverage divided by mean normalized coverage. If the desired coverage is 10x and the mean normalized coverage is 0.2, then the mean sequencing coverage is 50.
- 2
  Write out the equation used to calculate the amount of sequencing. This will look like (total targeted bases)(mean sequencing coverage)/(enrichment efficiency) = amount of sequencing.
- 3
  Plug in 62 MB for "total targeted bases" and 0.65 for the "enrichment efficiency." Add the sequencing coverage amount of 50x and the equation will look like (62)(50)/0.65. The result of the equation will be 4.8 GB of sequencing data.

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