Instructions
Look to see if the frog hops on top of other frogs, AllAboutFrogs.org advises. Males cling to the top of females to mate. However, the one at the bottom might not necessarily be a female, because male frogs can hop atop other male frogs. Females don't generally hop on top of other frogs.
Examine a frog's thumbs on the front legs during mating season. Males often develop pads called nuptial pads to help them grip onto the female frog when mating, AllAboutFrogs.org reports.
Locate the ears of the frog, called the tympanum. Located behind the eye, tympanums look like a circle. Bullfrogs, gray tree frogs, mink frogs and green frogs have smaller tympanums if they're female, the Minnesota Department of Natural Resources states. In most cases, if the tympanum in these frogs is no bigger than the eye, the frog is a female.
Check around the outside of the throat area. The frog is most likely a male if you see a pouch called a vocal sac, according to AllAboutFrogs.org. Male frogs typically fill the pouch with air from their lungs before making sounds, Encyclopaedia Britannica explains.
Observe the area of the throat. During mating season, the female frog may have a lighter color than the male frog, whose throat area gets darker, according to BullfrogControl.com. Some species of female frogs are easier to identify because they have a different throat color. The bullfrog female has a white or cream-colored throat, while the male bullfrog has a yellow throat area.