Instructions
Compare the sizes of fish within a species. Quite a few freshwater fish differ in size between the genders. For example, the female alligator gar --- a species of enormous, carnivorous fish native to the drainages of the American South --- tends to be larger than the male. The same pattern holds for the freshwater drum, while in many cichlid species it is the male that is the bigger sex.
Look for differences in body shape. The males of Pacific salmon species develop hooked jaws as they mature; the curved shape, contrasting with the straighter jaws of the females, becomes more pronounced as the adult salmon head upriver to spawn. In addition, during the same period, the male salmon often become humpbacked in appearance.
Watch for gender-distinguishing courtship behavior; the mating ritual, after all, is usually one of the surest ways to identify genders. The males of certain species of sunfish, for example, like the longear sunfish of the Mississippi and Great Lakes drainages, produces grunting sounds during courtship. They also assemble nests and then display in an attempt to convince prospective females to lay their eggs there.
Watch for gender-distinguishing parental behavior. You may see an adult fish take up its young inside its maw --- a parenting technique called "mouth-brooding." Depending on the species, the mouth-brooding parent may be the male or female; for example, in many cichlids, it is the female fish that displays this behavior.