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Multiple Isolation Techniques

Isolation techniques are essential for several types of examination that require samples to be separated from each other. This isolation allows researchers to study samples without interaction to other samples and to gather a better understanding of the traits of each sample and how those traits affect both the sample and the environment in which the sample exists. Isolation occurs in a variety of sciences, including chemistry, biology, computer science and electronics.
  1. Matrix Isolation Technique

    • Capturing free radicals or unstable substances, because of their nature, can be difficult because of the unpredictable nature of the substances. In such cases, a matrix isolation technique might be used. The matrix isolation technique aims to capture such substances in a solid matrix of inert matter. Imagine individual pieces of fruit isolated in a bowl of gelatin.

    Shallow Trench Isolation

    • Shallow trench isolation, also known as ̶0;box isolation,̶1; is employed in electronics. STI works by using an integrated circuit feature to prevent the leaking of any electrical current between adjacent semiconductors. During fabrication, a pattern of trenches is etched in the silicon then filled with silicon dioxide or another oxide material based on the application.

    Negative Room Pressure Isolation

    • Negative room pressure isolation works on the principle that areas of high pressure will naturally flow to areas of low pressure, wherein the low pressure area becomes the isolation area. This is valuable in areas such as hospitals, where cross-contamination poses health risks, and finishing facilities where toxic fumes are a necessary part of the job. In a household example, a bathroom exhaust fan creates negative pressure that draws air into the bathroom and pushes it out through a ventilation tube so that odorous air does not escape the bathroom.

    Gel Isolation

    • As one of the basic steps in genetic engineering, gel isolation, also known as gel extraction, isolates particular strands of DNA in agarose gel for litigation in further steps of an experiment. Gel isolation is noted by some professionals as being a difficult process that can be messy and create poor product yields. For its inconvenience, gel isolation is one of the most fundamental steps in gathering DNA strands.


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