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How to Grow Bacteria in NACL Solutions

While salt, or NaCl, is often used as a preservative to prevent the growth of bacteria in foods, some bacteria is resistant to the effects of NaCl, as long as it is in small quantities. The concentration that allows for bacteria to grow varies depending on the type of bacteria. Conduct an experiment to determine the maximum concentration of NaCl in which bacteria can grow and flourish. Through this experiment, you will learn which types of bacteria grow despite the presence of NaCl, as well as how much salt is too much.

Things You'll Need

  • Water
  • Petri dishes
  • Spoon or stir stick
  • Plastic or rubber gloves
  • Ultraviolet lamp
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Instructions

    • 1

      Heat up the water so it can dissolve the NaCl more quickly and thoroughly.

    • 2

      Fill each petri dish with water so each dish is almost full.

    • 3

      Add varying amounts of NaCl to each of the petri dishes, marking each one so you can track how much NaCl is in each dish. Concentrations should be 0.5 percent, 1 percent, 1.5 percent and 2 percent. Leave one petri dish without NaCl.

    • 4

      Stir the solution in each petri dish until the NaCl is completely dissolved.

    • 5

      Wear plastic or rubber gloves to protect yourself from the bacteria.

    • 6

      Add equal amounts of bacteria to each petri dish.

    • 7

      Position the petri dishes underneath an ultraviolet lamp to provide warmth for optimal bacteria growth.

    • 8

      Monitor the growth of the bacteria.


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