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Things You'll Need
Instructions
Grow Mold From Yeast
Obtain the yeast extract dextrose chloramphenicol agar. Most agar mediums are available in powder form and need to be mixed with water and boiled before adding to Petri dishes. Weigh 40 grams of the extract powder. Place the powder in one liter of distilled water. Soak the mixture for ten minutes. Stir and bring to boil on a hot plate. Dissolve the chloramphenicol X009 (X209) additive in ethanol and add to the extract mixture. Autoclave the mixture at 121 degrees C for 10 minutes. Cool to 45 degrees C.
Prepare at least four Petri Dishes using the cooled agar mixture. The prepared agar plates can be stored for up to seven days at 2 to 8 degrees C. Incubate the plates at 25 degrees C for five days. You do not need to inoculate the plates, because the agar mixtures are made specifically for the growth of certain organisms, in this case mold. If you do not have an incubator, you can place the Petri dishes in a room with the temperature at around 20 to 37 degrees Celsius. Cooler temperatures will also work; however, the cultures will take longer to grow.
Remove the plates from the incubator. Observe the plates for colonies. Colonies can be in bright colors. Use a mold identification book to determine the species of mold on your plate. There might be other types of organisms, such as bacteria, present on the plate as well. Record results. Take pictures as needed.