Things You'll Need
Instructions
Pour the water into the fermentation vessel. Dissolve the molasses into the water.
Place the pH tester in the solution. Add sodium carbonate to the solution. Stop when the pH of the water is 6.8.
Add the disodium hydrogen phosphate to the solution and mix manually with the stirrer.
Place the solution-filled fermentation vessel, along with the stirrer, air filter, airline tubing and aeration stone, into the steam sterilizer. Steam them at 212 degrees Fahrenheit for 45 minutes to pasteurize them. Allow the solution to cool back down to room temperature.
Connect the airline tubing to the aquarium pump at one end. Connect it to the aeration stone at the other end. Place the aeration stone into the solution and turn on the aquarium pump.
Add the inoculum culture to the solution. Mix the solution by setting the stirrer to stir the solution mechanically. Aerate and stir the solution for 30 minutes. Stop the stirring and aeration and remove the aeration stone. Allow the solution to continue fermentation on its own for another three to four hours.
Add roughly 1/3 cup of the sulfate. Watch the solution's fermentation become inhibited and allow the fermentation to begin again --- usually about half an hour to an hour later. Continue to add the sulfate in amounts of roughly 1/3 cup, using the same process until all of the sulfate is added --- roughly 27 to 33 hours.
Wait roughly 120 hours from the beginning of fermentation. Pour the solution through a filter to filter out the yeast. Add the sulfite-bisulfite solution. The remaining substance is roughly 0.7 oz. of pure glycerol.