Instructions
Lock onto one of the flower bombs on the floor, and send one of your minions to go pick it up with RT or R2 buttons. Move the minion over to one of the vents with the right analog stick.
Press the B or Circle button to recall the minion and it will drop the flower bomb on the vent. When the bomb explodes, it will release pollen and the Spider Queen will be lured toward it.
Run to the pressure switch and step on it with your overlord. This will pour water into the temple and knock the Spider Queen to the floor. Attack her until she recovers and climbs back onto the wall. You'll need to knock her to the ground a few more times to defeat her.